Figure 1.
Effect of IH on KDM proteins and activity in PC12 cells exposed to IH60 or normoxia (N). A, top: representative example of KDM and tubulin (loading control) protein expression in cell lysates from control (N) and IH-exposed PC12 cells. Bottom: densitometric analysis of the gels. B: KDM6 activity in nuclear lysates from control (N) and IH-exposed PC12 cells (means ± SE; n = 3 independent experiments run in duplicates). **P ≤ 0.05; ns = not significant (P > 0.05) as determined by one-way ANOVA test. IH, intermittent hypoxia; KDM, lysine demethylases; PC, pheochromocytoma.