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. 2021 Jul 23;53(9):385–394. doi: 10.1152/physiolgenomics.00045.2021

Figure 2.

Figure 2.

IH-augmented KDM6B protein in PC12 cells is independent of HIF-1α. A: representative example of HIF-1α and KDM6B protein expression with tubulin as loading control in total cell lysates from normoxia (N) and IH60-exposed cells treated with lentiviral scr shRNA or HIF-1α shRNA. B: densitometric analysis of gels (means ± SE; n = 3 independent experiments). C: KDM6B mRNA expression in lysates from PC12 cells treated with lentiviral HIF-1α shRNA or scr shRNA and exposed to 60 cycles of IH (IH60) or normoxia (N) (means ± SE; n = 3 independent experiments with triplicates). **P ≤ 0.05; ns = not significant (P > 0.05) as determined by Mann-Whitney test. HIF, hypoxia-inducible factor; IH, intermittent hypoxia; KDM, lysine demethylases; scr, scramble.