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. 2021 Jul 23;53(9):385–394. doi: 10.1152/physiolgenomics.00045.2021

Figure 4.

Figure 4.

KDM6B is required for IH-induced transcriptional activation of Nox4 in PC12 cells. A: KDM6 activity; B: KDM6A and KDM6B protein expression in nuclear lysates with TBP as loading control; and C: NOX4 mRNA in PC12 cells treated with GSKJ4 (50 µM), a specific KDM6 inhibitor, and exposed to IH60 or normoxia (N). D: KDM6 activity (means ± SE; n = 4 independent experiments run in duplicates); E: KDM6A and 6B protein expression with TBP as loading control (means ± SE; n = 3 independent experiments); and F: Nox4 mRNA in PC12 cells treated with lentiviral KDM6B siRNA or scr siRNA, and exposed to IH60 or normoxia (N) (means ± SE; n = 5). **P ≤ 0.05 as determined by one-way ANOVA. ns = not significant (P > 0.05). IH, intermittent hypoxia; KDM, lysine demethylases; Nox4, NADPH oxidases; PC, pheochromocytoma; scr, scramble; TBP, TATA-binding protein; veh, vehicle.