Figure 5.

KDM6B stimulates HIF-1α-mediated Nox4 transcription in PC12 cells. A: PC12 cells were exposed to IH₆₀ or normoxia (N) and then immunoprecipitated with anti-HIF1α antibody. Precipitates were separated on WES protein simple system and probed with anti-HIF-1α or anti-KDM6B. HIF-1α and KDM6B protein expression shown in black boxes. B: Nox4 enrichment determined by ChIP assay in the immunoprecipitates using antibodies against HIF-1α from PC12 cells treated with lentiviral KDM6B siRNA or scr siRNA, and exposed to IH₆₀ or normoxia (N). Data are presented as means ± SE from two independent experiments. **P ≤ 0.05; ns: not significant (P > 0.05) as determined by Mann-Whitney test. ChIP, chromatin immunoprecipitation; HIF, hypoxia-inducible factor; IH, intermittent hypoxia; KDM, lysine demethylases; Nox4, NADPH oxidases; PC, pheochromocytoma; scr, scramble.