Figure 6.
IH activates Nox4 through HIF-1/KDM6B in rat adrenal medulla (AM). Adult male rats were exposed to 10 days of IH along with daily treatment with either vehicle (IH10d) or GSKJ4 (10 mg/kg/day ip). Effect of GSKJ4 treatment on A: KDM6 activity; B: KDM6A and KDM6B protein expression; C: Nox4 mRNA; D: Nox activity; and E: H3K27me2 and me3 methylation status in cell lysates from AM. Data are means ± SE from 8 rats per group. **P ≤ 0.05; ns: not significant (P > 0.05). HIF, hypoxia-inducible factor; IH, intermittent hypoxia; KDM, lysine demethylases; N, normoxia; Nox4, NADPH oxidases; TBP, TATA-binding protein.