Table 3.

Effect of treatment (T), month (M), and interaction between treatment and month (T∗M) on fatty acid profile (FAP) in month 1 (M1) and month 3 (M3) of lactation.

	GTMR		GCHD		GTMR+P		SEM	p value
	M1	M3	M1	M3	M1	M3		T	M	T∗M
FA saturation (g/100 g of fat)
SFA	64.84	71.57	61.01	70.70	63.01	70.40	1.67	ns	<0.001	ns
MUFA	30.01	25.82	35.18	26.83	32.90	26.71	1.54	ns	<0.001	ns
PUFA	5.10Aa	2.60Ab	3.80Aa	2.46Ab	4.07Aa	2.87Ab	0.35	ns	<0.001	<0.001
n-3	0.31Aa	0.19ABa	0.41Aa	0.18Ab	0.43Aa	0.30Ba	0.04	ns	<0.001	0.01
n-6	3.66Aa	1.48Ab	2.09Ba	1.64Ab	1.94Ba	1.39Ab	0.19	ns	<0.001	<0.001
n6/n3	12.30Aa	8.52Ab	5.02Ba	8.95Ab	4.47Ba	4.72Ba	0.37	<0.01	ns	<0.001
Trans	4.93	1.87	4.14	2.12	5.37	3.16	0.50	ns	<0.001	ns
FA origin (g/100 g of fat)
De novo	24.72Aa	25.98Aa	18.23Aa	25.64Ab	19.84Aa	25.0Ab	1.90	ns	<0.001	0.02
(C4 : 0-C15 : 1)
Mixed origin	26.73	36.2	29.59	38.95	28.58	36.3	1.20	ns	<0.001	ns
(C16 : 0+C16 : 1)
Preformed	48.07	37.45	52.31	35.58	51.43	38.49	2.60	ns	<0.001	0.08
(>C17 : 0)

Treatments: GTMR: cows fed TMR; GCHD: cows diet change; GTMR+P: cows TMR+pasture. When there was significant interaction between treatment and month: differences between treatments in the same month (different capital letters) and difference between months within each treatment (different small letters) indicate p < 0.05 were shown. ns: no significance; SFA: saturated; MUFA: monounsaturated; PUFA: polyunsaturated; SEM: standard error of the mean.