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. 2022 Feb 22;2022:6293329. doi: 10.1155/2022/6293329

Figure 4.

Figure 4

NAR and Dox administration alone or in combination on the cell apoptosis in cardiomyocytes. (a) Flow cytometric dot plots (x-axis: Annexin V-FITC staining, y-axis: PI staining). (b) The H9c2 cells were analyzed for apoptosis using the TUNEL staining method. The panels display representative histological images (magnification×40). Scale bar = 100 μm. (c) Quantitative analysis of the fluorescence intensity. (d) Expression of cardiac apoptosis proteins in different treatment groups. Densitometric analysis of (e) caspase-3; (f) Bcl-2; and (g) Bax. The figure shows a representative blot for that protein. The values were expressed as fold changes over the level of β-tubulin, which served as a loading control. The results were analyzed using one-way ANOVA and Student t-tests. Data are presented as the mean ± SEM. Significance is indicated as P < 0.05. Versus the control, #P < 0.05; versus the Dox group, ∗P < 0.05.