Figure 5.

NAR antagonizes doxorubicin-induced cardiomyocyte apoptosis and oxidative stress by protecting mitochondria. (a) The ROS of NAR and Dox alone or in combination on H9c2 cardiomyocytes. (b) MDA level. (c) The images of the effect of doxorubicin, dexrazoxane, and NAR treatment on mitochondrial membrane potential in H9c2 cells (magnification×40). Scale bar = 100 μm. (d) Quantitative analysis of the fluorescence ratio of green fluorescence to red fluorescence is shown. (e) Cellular ATP concentration in H9c2 cells. Data were represented as mean ± SEM. ^#P < 0.05 compared to control; ^∗P < 0.05 compared to Dox.