Figure 5.

Diosmetin inhibited autophagy by enhancing the PI3K/Akt signaling pathway. (a) Representative western blot of autophagy-related protein LC3I/II p62/SQSTM1, Beclin1, and ATG7 in the heart. (c) Representative western blot of p-AKT, AKT, p-PI3K, PI3K, p-mTOR, mTOR, and GAPDH protein levels in the heart. (b, d) Quantitative of (a) and (c), respectively. (e) Representative confocal images showing colocalization of mCherry and EGFP signals in NRCMs transiently transfected with mCherry-GFP-LC3B adenovirus and treated as described above (one of 4 independent experiments), scale bar: 20 μm. Baf1: Bafilomycin A1. Data are presented as mean ± SEM. ∗p <0.05, ∗∗p < 0.01 vs. the sham group; #p <0.05, ##p < 0.01 vs. the AB group; ns: not significant.