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. 2022 Feb 22;2022:8367997. doi: 10.1155/2022/8367997

Figure 6.

Figure 6

Knocking down of p62 in NRCMs suppresses Nrf2 signaling pathway in response to diosmetin. (a) Western blots of p62 in NRMCs transfected with control (si-NC) siRNA or p62 siRNA (si-p62) for 48 h. (b) Quantitative of the protein level of p62. The statistical significance was evaluated by Student's t-test; ∗∗∗p < 0.001. (c) Western blotting for Nrf2, Keap1, Lamin B1, p62, and GAPDH protein levels in isolated NRCMs infected with indicated siRNAs. (d) Quantification of (c); GAPDH was used as a loading control, ∗p <0.05, ∗p < 0.01 vs. the PE group; #p <0.05, ##p < 0.01 vs. the PE+Dio group. (e) Effect of diosmetin on the mRNA level of antioxidant enzymes Gclc, Gsta1, Gstp1, and Nqo1 in NRCMs and mRNA level of Nrf2 and p62 as measured by qPCR, n = 6, ∗p <0.05, ∗∗p < 0.01 vs. the PBS group; #p <0.05, ##p < 0.01 vs. the PE group; $p <0.05, $$p < 0.01, and $$$p < 0.001 vs. PE+Dio. Representative fluorescent images (f) and quantitative analysis of ROS by ImageJ (g), scale bar: 50 μm. Data are presented as mean ± SEM, ∗∗p < 0.01. ns: not significant.