Figure 4.

FA reduces the ROS levels in 6-OHDA- or H₂O₂-induced BZ555 or N2 worms. (a) BZ555 worms treated with FA (50 μM) or L-Dopa (2 mM) for 72 h in the presence or absence of 6-OHDA (50 mM) were incubated with DHE solution (100 μM) for 1 h. Then, the representative images of worms with RFP signals were captured by using a fluorescence microscope. Scale bars: 20 μm. (b) The bar chart indicates the relative fluorescence intensity of DHE, which represents the ROS levels of BZ555 worms treated with 6-OHDA. Data were collected from at least 20 worms and were expressed as the mean ± SD. ^∗∗p ≤ 0.01. (c) N2 worms treated with FA (50 μM) or NAC (5 mM) for 72 h in the presence or absence of H₂O₂ (1 mM) were incubated with DHE solution (100 μM) for 1 h. Then, the representative images of worms with RFP signals were captured by using a fluorescence microscope. Scale bars: 20 μm. (d) The bar chart indicates the relative fluorescence intensity of DHE, which represents the ROS levels of N2 worms. Data were collected from at least 20 worms and expressed as the mean ± SD. ^∗∗∗p ≤ 0.001. (e) The survival rate of N2 worms treated with FA (50 μM) in the presence or absence of H₂O₂ (40 mM) was determined at different points. Data were collected from at least 60 worms for each treatment.