Figure 9.

FA inhibits 6-OHDA- or H₂O₂-induced apoptosis in PC-12 cells. (a, b) PC-12 cells were treated with FA or NAC in the absence or presence of 6-OHDA or H₂O₂ at the indicated concentrations for 24 h. After treatment, cell apoptosis was determined by PI/Hoehcst33324 staining. Representative images of cells with Hoechst or PI signals were captured by using a fluorescence microscope (magnification: 20x). Scale bar: 100 μm. The bar chart indicates the ratio of PI/Hoechst. Data were collected from three independent experiments and expressed as the mean ± SD. ^∗∗∗p ≤ 0.001. (c, d) The cell viability was measured by flow cytometry using the Annexin V-FITC/PI apoptosis detection kit after the treatment with FA or NAC in the absence or presence of 6-OHDA or H₂O₂. The bar chart indicates the percentage of viability of PC-12 cells. Data were collected from three independent experiments and expressed as the mean ± SD. ^∗∗∗p ≤ 0.001.