Table 1.
Kinetic parameters and average FPKM values of roots and MeJA-induced roots of SbUGTs and SbUGATs
| Enzymes | Km (μM) | Vmax (pkat mg protein− 1) | Vmax/Km | Average FPKM (Root) | Average FPKM (JA root) |
|---|---|---|---|---|---|
| SbUBGT | |||||
| SbUGT1 | 3.14 | 1027 | 327.07 | 12.53 | 28.18 |
| SbUGT2 | 100.60 | 44503 | 442.38 | 245.11 | 417.07 |
| SbUGT3 | 2.67 | 3964 | 1484.64 | 23.87 | 34.87 |
| SbUGT7 | – | – | – | 8.73 | 6.93 |
| SbUGT8 | – | – | – | 50.98 | 100.63 |
| SbUGT9 | – | – | – | 53.69 | 86.67 |
| SbUBGAT | |||||
| SbUGAT3 | 9.72 | 5607 | 576.85 | 2.38 | 2.63 |
| SbUGAT4 | 10.20 | 6266 | 614.31 | 222.29 | 674.07 * |
| SbUGAT5 | 15.44 | 8161 | 528.56 | 10.05 | 16.68 |
| SbUGAT6 | 12.32 | 7682 | 623.54 | 0.36 | 0.21 |
One asterisk (*) indicates a significant difference (0.01 < P < 0.05) between the root and MeJA-induced root under t-test. The products of SbUGT7, SbUGT8 and SbUGT9 were very low or not detected at the linear reaction stage