FIGURE 3.

Overexpression of IL‐35 induced cachexia and muscle wasting in SCID mice. (a–b) IL‐35 mRNA and protein levels were detected by RT‐PCR and ELISA in stable transfection cell lines. (c) Terminal peripheral blood samples were collected, and IL‐35 levels in serum measured. (d–e). A week after subcutaneous tumor transplantation, bodyweight and food intake were measured every day. (f–g) The gastrocnemius muscles was determined by hematoxylin and eosin staining (×20), and the cross‐sectional area (CSA) were quantitated. Results are shown as mean ± standard deviation