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. 2021 Nov 14;17(1):87–101. doi: 10.1016/j.ajps.2021.11.001

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© 2021 Shenyang Pharmaceutical University. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 3 — SA binds to Ca²⁺ and reduces the concentration of free Ca²⁺ in the damaged microenvironment. (A, B) Intracellular free Ca²⁺ was determined by Fura-2AM fluorescent probe and its statistical analysis. (C, D) Determination and statistical analysis of free calcium ion concentration in injured spinal cord tissue sections by Fura-2AM fluorescent probe. (E, F) Two-photon detection of free calcium ion concentration in hyaline spinal cord and its statistical analysis. All data represented the mean ± SD (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001.