Fig. 4.

NAL-SA-MVs inhibited the secretion of inflammatory mediators and achieved anti-inflammatory effect in vitro. (A) Fluorescence microscopy images for immunofluorescence staining of BV₂ cells were untreated, treated with PBS, NAL, SA-MVs and NAL-SA-MVs. Cell nuclei were stained with DAPI (blue), cell cytoskeleton was stained with β- tubulin (red), pro-inflammatory protein were stained with iNOS (green). (B) Fluorescence quantitative analysis in different groups. (C) Western blot detection of the inflammatory factors (iNOS, IL-6, IL-10) released by BV₂ cells in different groups. (D–F) Quantitative protein expression statistical analysis of (iNOS, IL-6 and IL-10 in figure C. All data represented the mean ± SD (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001.