NAL-SA-MVs inhibited the inflammatory response at the site of injuried spinal cord. (A) Western blot detection of the inflammatory factors (iNOS, IL-6, TNF-α, IL-1β, IL-10) from spinal cord tissue of different groups. (B-F) Quantitative protein expression statistical analysis of iNOS, IL-6, TNF-α, IL-1β, IL-10 in Fig. 5A. (G, H) Fluorescence microscopy images for immunofluorescence staining of spinal cord tissue sections. Cell nuclei were stained with DAPI (blue), pro-inflammatory protein were stained with IL - 1β (green). All data represented the mean ± SD (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001.