Fig. 6.

NAL-SA-MVs can inhibit the expression of apoptotic factors and has anti-apoptotic effect in vitro. (A, B) Representative flow cytometry analysis of the proportion of apoptotic cells in each treatment group and the corresponding quantitative analysis. (C, D) Fluorescence microscopy images of VSC4.1 cells were untreated and treated with PBS, NAL, SA-MVs, NAL-SA-MVs. Cell nuclei were stained with DAPI (blue), cell cytoskeleton was stained with β - tubulin (red), pro-apoptotic protein were stained with Caspase-3 (green). (E) Western blot detection of the apoptotic factors (Caspase-3, Bax, Bcl-2) released by VSC4.1 cells in different groups. (F–I) Quantitative protein expression statistical analysis of Caspase-3, Bax, Bcl-2 and Bax/Bcl-2 in figure E. All data represented the mean ± SD (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001.