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. 2021 Jun 8;43(3):659–671. doi: 10.1038/s41401-021-00689-2

Fig. 7. The effect of Epac activation on SOCS3 expression is mediated by C/EBP-β.

Fig. 7

a Immunofluorescence staining of C/EBP-β in HK-2 cells incubated with HG and treated with or without 8-O-cAMP (Scale bar = 20 μm). b Quantification of the MFI of C/EBP-β (n = 3). c Western blot analysis of C/EBP-β and SOCS3 in HK-2 cells under HG conditions that were treated with 8-O-cAMP or transfected with C/EBP-β siRNA. d, e Quantification of the Western blot results (n = 3). f SOCS3 mRNA expression in HK-2 cells cultured as described in c (n = 3). The values are the mean ± SD. *P < 0.05 vs LG; #P < 0.05 vs HG; &P < 0.05 vs HG + C/EBP-β siRNA.