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. 2021 May 2;43(3):552–562. doi: 10.1038/s41401-021-00675-8

Fig. 5. Functions of FABP7 in OLGs.

Fig. 5

a Western blot (WB) analysis of FABP3, FABP5, FABP7, and αSyn in the mouse brain and in KG-1C human oligodendroglial cells. The KG-1C cells endogenously express αSyn, FABP5, and FABP7. b Confocal microscopy of immunofluorescence staining for αSyn (red) and FABP7 (green) in KG-1C cells. In normal condition, FABP7 colocalizes with αSyn. c Cell viability analysis of KG-1C cells, based on CCK assay. Cells were cultured with psychosine for 24 h. Induced cell death was observed at a concentration of 10 µM (n = 6). d WB analysis of SDS-soluble fractions against αSyn (left) and FABP7 (right) reveals that 48-h incubation with FABP7 ligand 6 (1 µM) significantly reduced αSyn oligomer formation induced by psychosine (10 µM) in KG-1C cells. e WB analysis of Triton-soluble fractions against FABP7. f, g Quantification of αSyn and FABP7 oligomers in SDS-soluble fractions, based on WB analysis, is illustrated. FABP7 ligand 6 significantly reduced αSyn oligomer (n = 6) and FABP7 oligomer formation (n = 4). h Cell viability analysis of KG-1C cells, based on CCK assay. Cells were treated with ligand 6 (1 µM) which significantly improved KG-1C survival (n = 6). i Quantification of FABP7 monomer in Triton-soluble fractions, based on WB analysis, is illustrated. Endogenous FABP7 was significantly increased in the psychosine (20 µM) treatment group (n = 4). j Western blot (WB) analysis of αSyn, FABP7, and FABP5 showed that incubation with psychosine significantly enhanced αSyn formation, and treatment with PLA2 inhibitor blocked this effect (n = 4). k PLA2 activities of KG-1C cells following psychosine treatment (10 µM), with and without PLA2 inhibitors (10 µM) using PLA2 activity assay (n = 4). l, m The quantification of αSyn and FABP7 oligomers in SDS-soluble fractions, based on WB analysis, is illustrated. The data are shown as mean ± standard error of the mean and were obtained using one-way analysis of variance. *P < 0.05; **P < 0.01; #P < 0.05; ##P < 0.01. ɑSyn alpha-synuclein, CCK cell counting kit-8, FABP3, 5, and 7 fatty acid-binding protein 3, 5, and 7, NS not statistically significant, SDS sodium dodecyl sulfate.