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. 2021 May 20;43(3):703–711. doi: 10.1038/s41401-021-00673-w

Fig. 1. SQSTM1 interacts with AKR1C1.

Fig. 1

a NCI-H1299 cells were transfected with AKR1C1-Flag or AKR1C1. Cells were subjected to Flag IP and assessed by LC-MS/MS. Venn diagrams were used to visualize the potential AKR1C1-interacting proteins (left). Vertical bars represent proteins (unique peptides ≥ 2) from both batch 1 and batch 2 according to the rank of protein scores (right). b GST pulldown assay was utilized to evaluate the interaction of recombinant GST-AKR1C1 with SQSTM1 from whole cell lysates of NCI-H1299. c AKR1C1-HA or SQSTM1-Myc or their combination were expressed in 293T cells for 36 h. Myc IP and IB were applied to the indicated cells. d Vector or SQSTM1-Myc plasmids were introduced into NCI-H460 for 36 h. Cells were subjected to Myc IP and IB.