Fig. 5. Effects of QF84139 (QF) against PE-induced myocyte hypertrophy are blocked by compound C (CpC) in vitro.

The NRCMs were treated with CpC for 1 h before addition with metformin (MET, 1 mM) or QF (3 μM), and then stimulated with PE (10 μM) for 48 h. a Schematic diagram of different treatments and analysis. qRT-PCR analysis of the mRNA expression of ANP (b), BNP (c) and β-MHC (d), n = 6. e Representative images (upper panels) and averaged data (lower panels) for immunoblot analysis of ANP in NRCMs. n = 5. Representative images (f) and averaged cell size (g) for immunofluorescence staining with anti-α-actinin antibody (red), anti-ANP antibody (green), and DAPI (blue) in NRCMs (200 random cells/heart, n = 3 hearts per group). Scale bar = 10 μm. *P < 0.05, **P < 0.01, ***P < 0.001. n.s., nonsignificant