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. 2022 Mar 1;5:182. doi: 10.1038/s42003-022-03133-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a LNCaP, C4-2, VCaP and PC-3 cells were infected with shScramble or shOTUD6A, and cell growth ratio were examined by SRB assays. Data were shown as mean ± SD of three independent experiments. ***P < 0.001. IB analysis results of the whole cell lysates (WCLs) were shown on the top of curves. b Representative images of the colony formation assay of LNCaP, C4-2, VCaP, and PC-3 cells infected with shScramble or shOTUD6A. Scale bars: 5 mm. The statistic results were shown as mean ± SD of three independent experiments. ***P < 0.001. shScr: shScramble; sh6A#1: shOTUD6A#1; sh6A#2: shOTUD6A#2. c, d IB analysis (c) and colony formation (d) images of C4-2 cells transfected with the indicated plasmids. WT: wild-type. Scale bars: 5 mm. e, f Stable C4-2-shScramble or C4-2-shOTUD6A cells were randomly injected subcutaneously into 6-week-old NOD/SCID mice and then tumor growth curves were constructed (e), and dissected tumors were collected and weighed (f) around 5 weeks later (n = 6). Data represented the mean ± SEM. Statistical significance was determined by two-tailed unpaired Student’s t-test, **P < 0.01, scale bar: 1 cm. g, h Indicated stable Myc-CaP cells were randomly injected subcutaneously into 6-week-old FVB mice and then tumor growth curves were constructed (g), and dissected tumors were collected and weighed (h) 4 weeks later (n = 6). Data represented the mean ± SEM. Statistical significance was determined by two-tailed unpaired Student’s t-test, **P < 0.01, ***P < 0.001, scale bar: 1 cm. 6A WT: wild-type OTUD6A; 6A C153A: OTUD6A-C153A mutant.