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. 2022 Mar 1;5:182. doi: 10.1038/s42003-022-03133-1

Fig. 6. OTUD6A erases K27-linked polyubiquitination of Brg1 and K11-linked polyubiquitination of AR.

Fig. 6

a–d IB analysis of WCLs and IPs derived from 293T cells transfected with the indicated HA-Ub-mutants together with Flag-Brg1 or Flag-AR with or without Myc-OTUD6A. e–g IB analysis of WCLs and IPs in 293T cells transfected with the indicated plasmids. h Quilt plot depicting OTUD6A, FBXW7, and SPOP gene alterations, including amplification, mutation and deep deletion, in combined prostate datasets (SU2C [2019, 444 samples], SU2C [2015, 150 samples] and FHCRC [2016, 176 samples]) (http://www.cbioportal.org/). i Heatmap of altered genes in PC-3-shScramble, PC-3-shOTUD6A or PC-3-shBrg1 cells by RNA-seq analysis. The right panel showed the Venn diagrams of number of identified genes in different groups. shScr: shScramble. j The proposed model for OTUD6A in regulating tumorigenesis in PCa.