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. 2022 Mar 1;5:185. doi: 10.1038/s42003-022-03132-2

Fig. 1. Assessing endosomolytic compounds for their ability to increase functional SSO activity.

Fig. 1

a Chemical structures of CMP01, CMP05, and CMP05-7. b Functional SSO activity during co-treatment with chosen compounds. HeLa_Luc705 cells were pre-treated with 1 µM SSO, and then co-treated with 10 µM, 5 µM, or 1 µM of the compound for 2 h . Cells were then rinsed and incubated for 4 h in growth media. Data come from N = 3 experiments, each with n = 3 biological replicates. Values are reported as fold increase over activity from 1 µM naked SSO. Significance was determined (excluding LF2000) by two-way ANOVA followed by Dunnett’s posttest to the oligo only control where ****P < 0.0001, ***P < 0.001, *P < 0.05, and n.s = not significant. c Functional SSO activity during co-treatment with CMP05 analogs. HeLa_Luc705 cells were exposed to 5 µM of the compound and either 1 µM SSO or 100 nM SSO for 2 h. Cells were then rinsed and incubated for 4 h in growth media. Values are reported as fold increase over activity from 30 h treatment with 1 µM naked SSO. Data comes from n = 3 experiments. Significance was determined (excluding LF2000) by two-way ANOVA followed by Dunnett’s post test to the oligo only control where ****P < 0.0001 and n.s = not significant. Intraexperimental variability can contribute to discrepancies in fold increase between experiments.