Fig. 5. STORM imaging of SSO trafficking in CMP05-treated HeLa and HuH7 cells.
a Diffraction-limited and STORM images of lysosomes (red; LAMP1 ICC) and Alexa-Fluor 488-labeled SSO (green) in control HeLa cells and after 2 h treatment with 5 µM CMP05. Scale bars 5 µm, except in the zoomed area 1 µm. b Diffraction-limited and STORM images of lysosomes (red; LAMP1 ICC) and Alexa-Fluor 488-labeled SSO (green) in control HuH7 cells and after 2 h treatment with 2.5 µM CMP05. Scale bars 5 µm, except in the zoomed area 1 µm.