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. 2022 Mar 1;5:185. doi: 10.1038/s42003-022-03132-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Live-cell confocal microscopy reveals a simultaneous decrease of SSO-containing bodies and Gal9 translocation. HuH7_mCherry-Gal9 and HeLa_mCherry-Gal9 were pre-loaded with SSO-488 (green) for 24 h and then treated with various concentrations of CMP05 and Hoechst to visualize nuclei (blue). Gal9 events appear as yellow puncta. Scale bars = 20 µm (larger panels) and 5 µm (insets). White boxes indicate inset regions. b Quantitative analysis of cells treated as in a and imaged every 2.5 min. Analysis reveals a time and dose-dependent decrease in the total number of SSO-488 structures per cell. Data represent mean −/+ SEM from n = 3 independent experiments. c Cells treated as in (b) and quantitation of mCherry-Gal9 puncta formation. Analysis displays a dose and time-dependent increase of Gal9 structures per cell. Data represent mean −/+ SEM from n = 3 independent experiments. Significance was determined by two-way ANOVA followed by Dunnett’s posttest to the DMSO control where ****P < 0.0001 and n.s = not significant.