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. 2022 Mar 1;5:185. doi: 10.1038/s42003-022-03132-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a–c Live-cell microscopy of HuH7 cells overexpressing mRFP-Rab5 (a), mRFP-Rab7 (b) or Lamp1-RFP (c) after 15 min pulse-incubation with 1 µM SSO and 2.5 µM CMP05. The cells were continuously exposed to 2.5 µM CMP05 and imaged over time. The images were analyzed for colocalization between 488-SSO and (m)RFP by the ComDet plugin to ImageJ and detected particles are marked with a circle (green for 488-SSO, red for (m)RFP and yellow for colocalized particles). Scale bars are 20 µm in the full images and 5 µm in the zoomed images. d Quantitation of (m)RFP colocalized with 488-SSO using the ComDet plugin. The data are presented as the percentage of detected (m)RFP-positive vesicles colocalized with SSO-positive vesicles ± SD (CMP05: Rab5 n = 4, Rab7 n = 4, Lamp1 n = 5; DMSO: all cell lines n = 6).