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. 2022 Mar 1;5:180. doi: 10.1038/s42003-022-03127-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Brightfield images showing the extraction of cytoplasmic fluid from C. cinerea. Upon application of suction, the cellular fluid is collected in the front of the FluidFM probe (inserts). The targeted hyphae was growing until suction was applied for extraction (blue arrows). Vacuoles are visible after the extraction, exclusively within the targeted compartment. b Extraction (0 h) and subsequent monitoring of the dTomato fluorescent reporter. c Quantified dTomato fluorescence intensity of individual hyphal compartments over time after extraction. Red curves profiles were obtained from extracted hyphal compartments (N = 5). Gray curves are dTomato expression profile from control, non-perturbed hyphal compartments within the same mycelia (N = 5). Scale bars: 20 µm.