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ESR1-SLC7A11 axis is involved in IR-induced ferroptosis. The results of Western blot assay show that ESR1 knockdown inhibited the increase of SLC7A11 induced by IR at 12 h in MCF-7 (A) and ZR-75-1(F). (B–E,G–J) Lipid peroxidation and cell death were assessed by flow cytometry using C11-BODIPY and trypan blue, respectively. Shown is mean ± SD, n ≥ 3, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
