Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 16;9:772380. doi: 10.3389/fcell.2021.772380

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Liu, Liu, Bian, Zhang, Wang, Chen, Jiang, Li, Chen, Xue, Li, Liu, Liu and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

SLC7A11 is degraded by ubiquitination after IR treatment. MCF-7 (A) and ZR-75-1 (B) cells were pretreated with MG132 (10 μM) or DMSO for 4 h before harvest. (C) MCF-7 cells were transfected with Si-NEDD4L. The protein level of SLC7A11 was analyzed by Western blot. (D) Western blot analysis of WCL and IP samples of IgG or anti-NEDD4L antibody were conducted to detect the interaction between SLC7A11 and NEDD4L. MCF-7 cells were transfected with FLAG-SLC7A11 for 48 h, then treated with IR. MG132 (10 μM) was added into the culture medium for 4 h before harvest. (E) Western blotting analysis of WCL and ubiquitin IP samples were performed with indicated antibody in MCF-7 cells. The cells were treated with MG132 (10 μM) for 4 h before harvest.