TABLE 1.

Primer sequences and annealing temperatures used in real-time RT-PCR assay for energy metabolism related gene targets.

Gene	NCBI Genbank ID	FW primer sequence	RV primer sequence	Tm (°C)
apoa1a	NM_131128.1	GAA​GGC​CTT​CGA​GTC​CAA​CA	TCT​GTG​CCG​AAT​GTG​GTC​CTC	55
apoba	XM_689735.9	AGC​TGA​AGA​ACG​CAC​TCT​CC	GAA​CTT​CAG​GGC​CGC​ATC​TA	57
insa	NM_131056.1	TAA​GCA​CTA​ACC​CAG​GCA​CA	GAT​TTA​GGA​GGA​AGG​AAA​CC	59
insb	NM_001039064.1	ACT​CTT​CAC​AGA​CTC​TGC​TC	ACA​GAT​GCT​GGG​ATG​GAG​AA	59
pck	NM_214751.1	GCA​CGG​AGT​GTT​TGT​AGG​G	GGT​CTC​GGT​TCA​GTT​CAC​G	56
pomca	NM_181438.3	GCC​CCT​GAA​CAG​ATA​GAG​CC	CTC​GTT​ATT​TGC​CAG​CTC​GC	54
pomcb	NM_001083051.1	TCC​ATC​GAG​CTC​CAA​AAC​CC	ACATTTTACGGTCTGCGT	54