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. 2022 Feb 16;13:821803. doi: 10.3389/fmicb.2022.821803

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Copyright © 2022 Kearney, Coe, Castro and Chisholm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree of the 16S-18S internal transcribed spacer (ITS) sequence (for molecular methods, see Rocap et al., 2002; MUSCLE Edgar, 2004 was used to generate the ITS alignment, and FastTree Price et al., 2010 to generate the tree, sequences are available at https://doi.org/10.6084/m9.figshare.17076446) for Synechococcus isolates obtained from natural seawater using the filter plating technique described here (red), with previously isolated Synechococcus strains (purple), annotated with their lineages. Strains are colored by subcluster. Prochlorococcus str. MIT9313 ITS (green) sequence serves as the outgroup to root the tree.