Figure 2.

Production of murine CAR T cells that recognize murine CD19 and analysis of their ability to kill 4T1-mCD19 cells in vitro. (A) Spleen derived T cells were transduced to express an anti-CD19 CAR and then examined for transduction efficiency by flow cytometry using an anti-rat IgG labeled with AlexaFluor 594, as described in Methods. Data shown are representative of three separate transduction procedures. (B) Analysis of the ability of the aforementioned anti-mouse CD19 CAR T cells to kill 4T1-mCD19 cancer cells in vitro. (Effector:target cell ratio = 5:1). Bar graphs display mean ± S.D. n=3.