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. 2022 Feb 16;13:816761. doi: 10.3389/fimmu.2022.816761

Figure 5.

Figure 5

Impact of CAR T cells and FA-TLR7-1A on the properties of tumor infiltrating TAMs and MDSCs. (A) Estimation of the M1:M2 ratios of F4/80 positive macrophages within the solid 4T1-mCD19 tumors following treatment with the indicated therapies. The ratio of inducible nitric oxide synthase (iNOS) to arginase 1 (Arg 1) was used as an estimate of M1-like to M2-like macrophages. (B) Percentage of live CD45+ cells in the solid tumors that were F4/80 positive macrophages. (C) Percentage of MDSCs in live CD45+ cells in the solid tumors. (D) Ratio of the iNOS+ to Arg 1+ population among live MDSCs. (E) Percentage of the iNOS+ population in live MDSCs. (F) Percentage of Arg 1+ population in live MDSCs. This study was performed three times with similar results each time. The number of mice/group is the same as reported in the legend to Figure 3 , except in the CAR-T + 3 nmol FA-TLR7-1A, 5/W group only 5 of 9 tumors could be analyzed because 4 of 9 mice experienced complete cures. Mean ± SEM. Statistical significance was determined using an unpaired two‐tailed t‐test (*P < 0.05, ***P < 0.001, ns, not significant P ≥ 0.05).