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. 2022 Feb 16;13:780945. doi: 10.3389/fimmu.2022.780945

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Copyright © 2022 Chicana, Abbasizadeh, Burns, Taglinao, Spencer and Manilay

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bone marrow, spleen and peripheral blood lineage cell defects in the VhlcKO mice. (A) Macroscopic and ex vivo imaging of the distal end of long bones revealed progressive increases in the bone mass of 10-weeks-old VhlcKO femurs compared to control. Inset: photo of the femur. Red: blood vessel (AlexaFluor647 CD31, AlexaFluor647 CD144, AlexaFluor647 Sca-1), Blue: bone (SHG). Scale bar ~500μm; (B) bone marrow cellularity, (C) representative FACS plots of immune cell lineages, with the values on plots on the left representing cell frequency in total bone marrow, and the values on the plots on the right representing frequencies within the Gr-1- CD11b- gate. (D) frequency analysis of bone marrow lineage cells at 6-weeks of age (left), 10-weeks of age (middle) and 6-month (right). p<0.05*, p<0.01**, p<0.001***, p<0.0001**** two-tailed Student’s t-test. N.S., not statistically significant.