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. 2022 Feb 16;13:780945. doi: 10.3389/fimmu.2022.780945

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Copyright © 2022 Chicana, Abbasizadeh, Burns, Taglinao, Spencer and Manilay

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dysregulated B cell development in VhlcKO mice bone marrow. (A) Antigen markers used to identify and distinguish B cell development stages by flow cytometry. (B) Representative FACS plot of B cell development in the BM control (top) and VhlcKO (bottom); (C) B cell frequency in 6-week-old (left), 10-week-old (middle), and 6-month-old (right) mice; (D) absolute cell numbers in 6-week-old (left), 10-week-old (middle), and 6-month-old (right). p<0.05*, p<0.01**, p<0.001***, p<0.0001**** two-tailed Student’s t-test.