Figure 5.

VhlcKO mice display increase apoptosis and reduced cell proliferation during early B cell development. (A) Representative FACS plots of apoptotic phases (live, early apoptosis, and late apoptosis) in B220+ cells (left) and cell cycle phases (G0 (quiescent), G1, and S/G2/M) in B220+ cells (red:CD43+ blue: CD43-) (right) in 10-weeks-old mice; (B) frequency of apoptotic phases in Fractions A and B-C in 6-weeks-old, 10-weeks-old and 6-month-old mice; (C) frequency of cells in each cell cycle phase within Fractions A and B-C at 6-weeks-old, 10-weeks-old old and 6-month-old mice. Our cell cycle analysis antibody panel ( Supplementary Table 1 ) did not include anti-IgM and anti-IgD, so we could not remove these cells from the CD43+ population. However, these cells are very low in frequency in the VhlcKO ( Figure 3 ) and do not significantly change the proliferation results in controls (data not shown); (D) CXCL12, SCF, TPO, and EPO cytokine level measurements in bone marrow supernatant of combined 10-weeks-old (filled) and 6-months-old (open) control or VhlcKO mice. p<0.05*, p<0.01** two-tailed Student’s t-test. N.S., not statistically significant.