Figure 6.
Motor neuron-specific SOD-1 modulates the density of glutamate receptor at the ventral nerve cord
(A) Fluorescence micrographs of glr-1p:glr-1:GFP in wild-type N2, sod-1(tm776), sod-1(tm776) + sod-1p:sod-1:mRFP and sod-1(tm776) + unc-129p:sod-1:mRFP backgrounds. Arrow indicates GLR-1:GFP puncta. Scale bar indicates 10μm.
(B) Cholinergic motor neuron expression of SOD-1 rescues the GLR-1:GFP density phenotype of sod-1(tm776) deletion. Error bar represents SEM ∗∗∗ represents p < 0.001. ∗∗∗∗represents p < 0.0001. ns represents not significant. As determined by one-way ANOVA, followed by Tukey's multiple comparison analysis. N = 20.
(C) AMPA-type glutamate receptor GLR-1 promotes C. elegans avoidance response to P. aeruginosa PA14 via inhibition to SOD-1. SOD-1 modulates synaptic GLR-1 density at the ventral nerve cord and plays a role in glr-1 dependent spontaneous reversal.
