Figure 3.

Liver-derived pEV factors in AD brain tissue.

(a) Staining of CP brain sections for CRP and haptoglobin from one age-matched control (H-08) and 3 AD patients (AD-12-14). (b) Quantification of CP cell staining (pixel/cell) for CRP and haptoglobin by imaging software. SDM based on 3 visual areas analyzed (40 cells/area). (c) Representative image of mouse CP cells incorporating Al₃-induced EV. (d) Representative images of a multiantigen analysis on human tissue (AD13), showing that pEV markers and CRP co-localize in the CP. (e) Representative images of CRP staining in hippocampal brain sections of one control (Con-01) and one AD patient (AD-13) and quantification of positive cells in hippocampal and cortical areas (3 controls and 3 AD patients (see also Fig. S5b). SDM based on 100 cells analyzed in each sample. Statistical significance for (d) and (f) by two-way ANOVA and Dunnett's test, ***p < 0.005.