Figure 7.

Potentiated Hsp104 variants suppress the toxicity of MATR3 pathogenic mutants

(A) W303Δhsp104 yeast were sequentially transformed with pAG423GAL-MATR3-GFP and vector, pAG416GAL-Hsp104, or the indicated variants. The strains were serially diluted 5-fold and spotted on glucose (non-inducing) or galactose (inducing) media. Scores are based on number of spots from an average of at least three replicates.

(B) Strains from A were induced for 5h, lysed, and immunoblotted for MATR3 (GFP, mouse), Hsp104, and PGK (loading control). Certain immunoblots were cropped to remove additional lanes for presentation purposes, and crop lines are marked with lines.