REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Hsp104	Enzo	ADI-SPA-1040
Mouse monoclonal anti-PGK	Invitrogen	459250
Rabbit monoclonal anti-MATR3 N-terminus	Abcam	Ab151739
Rabbit polyclonal anti-GFP	Sigma	G1544
Mouse monoclonal anti-GFP	Roche	1184-460-001
Goat anti-rabbit secondary antibody	Li-cor	926-68071
Goat anti-mouse secondary antibody	Li-cor	926-32210
Bacterial and virus strains
XL10-Gold cells	Agilent	230245
One Shot TOP10 Chemically competent E. coli	Invitrogen	C404010
Chemicals, peptides, and recombinant proteins
PfuUltra II Fusion HS DNA Polymerase	Agilent	600672
DpnI	New England Biolabs	R0176S
BP clonase	Invitrogen	11789020
LR clonase	Invitrogen	11791020
Experimental models: Organisms/strains
S. cerevisiae: W303a (MATa, can1-100, his3-11, 15, leu2-3, 112, trp1-1, ura3-1, ade2-1)	Schirmer et al. (2004)	N/A
S. cerevisiae: W303aΔhsp104 (MATa, can1-100, his3-11, 15, leu2-3, 112, trp1-1, ura3-1, ade2-1, hsp104::KanMX)	Schirmer et al. (2004)	N/A
S. cerevisiae: W303aΔhsp104-pAG303GAL-TDP-43-YFP	Aaron Gitler	N/A
S. cerevisiae: W303a-pAG303GAL-FLAG-103Q-GFP	James Shorter	N/A
Recombinant DNA
MATR3 cDNA	Dharmacon	MHS6278-202757255
pDONR221-ccdB	Invitrogen	12536017
pAG416GAL-ccdB	Alberti et al. (2007)	N/A
pAG413GAL-ccdB	Alberti et al. (2007)	N/A
pAG423GAL-ccdB-EGFP	Alberti et al. (2007)	N/A
pAG303GAL-ccdB-EGFP	Alberti et al. (2007)	N/A
pDONR-221-MATR3 WT	This paper	N/A
pDONR-221-MATR3:S85C	This paper	N/A
pDONR-221-MATR3:F115C	This paper	N/A
pDONR-221-MATR3:P154S	This paper	N/A
pDONR-221-MATR3:T622A	This paper	N/A
pDONR-221-MATR3 WT (no stop codon)	This paper	N/A
pDONR-221-MATR3:S85C (no stop codon)	This paper	N/A
pDONR-221-MATR3:F115C (no stop codon)	This paper	N/A
pDONR-221-MATR3:P154S (no stop codon)	This paper	N/A
pDONR-221-MATR3:T622A (no stop codon)	This paper	N/A
pAG413GAL-MATR3 WT	This paper	N/A
pAG413GAL-MATR3:S85C	This paper	N/A
pAG413GAL-MATR3:F115C	This paper	N/A
pAG413GAL-MATR3:P154S	This paper	N/A
pAG413GAL-MATR3:T622A	This paper	N/A
pAG423GAL-MATR3-eGFP	This paper	N/A
pAG423GAL-MATR3:S85C-eGFP	This paper	N/A
pAG423GAL-MATR3:F115C-eGFP	This paper	N/A
pAG423GAL-MATR3:P154S-eGFP	This paper	N/A
pAG423GAL-MATR3:T622A-eGFP	This paper	N/A
pDONR-221-MATR3(1-397)	This paper	N/A
pDONR-221-MATR3(1-575)	This paper	N/A
pDONR-221-MATR3(398-595)	This paper	N/A
pDONR-221-MATR3(398-847)	This paper	N/A
pDONR-221-MATR3(576-847)	This paper	N/A
pAG423GAL-MATR3(1-397)-eGFP	This paper	N/A
pAG423GAL-MATR3(1-575)-eGFP	This paper	N/A
pAG423GAL-MATR3(398-595)-eGFP	This paper	N/A
pAG423GAL-MATR3(398-847)-eGFP	This paper	N/A
pAG423GAL-MATR3(576-847)-eGFP	This paper	N/A
pAG423GAL-MATR3Δ398-473-eGFP	This paper	N/A
pAG423GAL-MATR3Δ496-575-eGFP	This paper	N/A
pAG423GAL-MATR3Δ588-595-eGFP	This paper	N/A
pAG423GAL-MATR3Δ398-473, Δ496-575-eGFP	This paper	N/A
pAG416GAL-Hsp104	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:A503V	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:A503S	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:A503G	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:V426L	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:A437W	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:N539K	Jackrel et al. (2014)	N/A
pAG416GAL-Hsp104:Y257F-A503V-Y662F	Jackrel et al. (2014)	N/A
Software and algorithms
Image J	Schneider et al. (2012)	https://imagej.nih.gov/ij/
GraphPad Prism	GraphPad	Version 9.0