Bacterial and virus strains |
|
Plasmid: pCas9 |
Addgene |
Cat:42876 |
|
Chemicals, peptides, and recombinant proteins |
|
Oleic acid |
TCI |
CAS: 112-80-1 |
palmitoleic acid |
TCI |
CAS: 373-49-9 |
Stearic acid |
TCI |
CAS: 57-11-4 |
Palmitic acid |
TCI |
CAS: 57-10-3 |
DHA |
TCI |
CAS: 6217-54-5 |
Trizol |
Ambion |
Cat:15596-026 |
EPA |
TCI |
CAS: 10417-94-4 |
|
Critical commercial assays |
|
PrimeScript™ RT Reagent Kit |
TaKaRa |
Cat:RR047A |
|
Deposited data |
|
SRA RNA-seq data |
This paper |
PRJNA770361 |
|
Experimental models: Organisms/strains |
|
AB strain |
Our lab |
N/A |
Scd5-KO |
This paper |
N/A |
|
Software and algorithms |
|
GraphPad Prism |
Prism |
https://www.graphpad.com/scientific-software/prism |
Amira |
Thermo Fisher Scientific |
https://www.thermofisher.cn/cn/zh/home/industrial/electron-microscopy/electron-microscopy-instruments-workflow-solutions/3d-visualization-analysis-software/amira-life-sciences-biomedical/amira-cell-biology.html |
CLUSTALW |
Kyoto University Bioinformatics Center |
https://www.genome.jp/tools-bin/clustalw |
Adobe Illustrator |
Adobe |
https://www.adobe.com/cn/products/illustrator.html |
Image J |
Schneider et al. (2012) |
https://imagej.nih.gov/ij/ |
|
Oligonucleotides |
|
Primers for qPCR, see Table S3
|
This paper |
N/A |