Figure 1.

Clustering scRNA-seq data from E11.5 mouse lungs reveals expected cell populations

(A) Sample image of a lung used for scRNA-seq experiment. Each lung was micro-dissected to isolate the left lobe (indicated by dotted outlines). Scale bar shows 100 μm.

(B and C) UMAP plots of cell cycle-corrected embryonic lung cells isolated by scRNA-seq of E11.5 CD1 mouse lungs, color-coded either by cluster (B) or by replicate (C).

(D) UMAP plots from (B and C) color-coded to show the expression of population-specific genes.

(E) Top 20 markers for each cluster shown in (B) based on log fold-change (FC) and color-coded by adjusted p value.