Table 2.

Oocyte and embryo developmental stage, method of collection and sequencing for DNA methylation

Specie	Stage	Collection	Sequencing method	Equipment	Reference
Bovine	MII, 2, 4, 8 and 16C	In vivo	WGBS	Illumina HiSeq4000	(Duan et al., 2019)
Bovine	MII, 2C, 4C 8C, 16C and BL	In vitro	post-bisulfite adapter tagging (PBAT)	Illumina HiSeq 1000	(Ivanova et al., 2020)
Bovine	MII, 2, 4, 8C, BL	In vivo	Reduced representation bisulfite sequencing (RRBS)	Illumina Hiseq2500	(Jiang et al., 2018)
Human	MII, 2, 4, 8C, MO, ICM and TE	In vitro	Single-cell whole-genome bisulfite sequencing WGBS)	Illumina HiSeq 2500 or HiSeq 4000	(Zhu et al., 2018)
Human	MII, 2, 4, 8C, MO and ICM	In vitro	WGBS	HiSeq2000 sequencer	(Guo et al., 2014)
Mouse	MII	In vivo	WGBS were prepared using the PBAT method	Illumina HiSeq 2000	(Shirane et al., 2013)
Mouse	MII and 2C	In vivo	MethylC-Seq	Illumina HiSeq 2000	(Wang et al., 2014)
Mouse	2, 4, 8C and ICM	In vitro	Reduced representation bisulfite sequencing	Illumina Genome Analyzer IIx	(Smith et al., 2012)
Mouse	MII, 2, 4, 8C, MO, ICM and TE	In vivo	Single-cell multi-omics sequencing technology (single-cell COOL-seq)	Illumina HiSeq 2500	(Guo et al., 2017)