Skip to main content
. 2022 Jan 30;298(3):101666. doi: 10.1016/j.jbc.2022.101666

Figure 1.

Figure 1

Intracellular activity of GnT-V WT and GnT-VΔN.A, schematic model of the GnT-V catalyzed reaction on N-glycan. B, the three-dimensional structure of the human GnT-V luminal region (left) and N domain (right). L189 (which corresponds to L188 of hamster GnT-V) is shown. C, plasmid constructs of GnT-V WT and GnT-VΔN. TM stands for transmembrane region. D, N-acetylglucosaminyltransferase-V WT or GnT-VΔN was expressed in HeLa GnT-V KO cells. The cellular proteins were separated by SDS-PAGE, followed by CBB staining or blotting with anti-GnT-V, anti-GAPDH, or L4-PHA lectin. E, left, FACS analysis of HeLa GnT-V KO cells with L4-PHA lectin. Dotted line, nonstained cells; solid line, stained mock-transfected cells; colored with light blue, GnT-V WT-expressing cells; colored with orange, GnT-VΔN-expressing cells. Right, the relative geometric means of L4-PHA-FITC signals are shown in the graph (n = 3). Three independent assays were carried out using different sets of transfected cells. F, N-acetylglucosaminyltransferase-V WT or GnT-VΔN was expressed in HEK293 GnT-V KO cells. The cellular proteins were separated by SDS-PAGE, followed by CBB staining or blotting with anti-GnT-V, anti-GAPDH, or L4-PHA lectin. GnT-V, N-acetylglucosaminyltransferase-V; L4-PHA, leukoagglutinating-phytohemagglutinin.