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. 2022 Jan 29;298(3):101614. doi: 10.1016/j.jbc.2022.101614

Figure 5.

Figure 5

mtm-10 mutation enhances susceptibility to pathogen-induced dendrite degeneration and decreases the lifespan.A representative survival plot of control CX5974 (parent strain), mtm-10(ac270), mtm-10(ok2711), mtm-10(ac270);Pmtm-10::mtm-10, and mtm-10(ac270);Podr-1::mtm-10::SL2::GFP animals on full lawns of Pseudomonas aeruginosa (independent replicates N = 3, number of animals n = 200), control versus mtm-10 (ac270)(n.s.). B, representative lifespan plots of control CX5974 (parent strain), mtm-10(ac270), mtm-10(ok2711), and rescued strains mtm-10(ac270); Pmtm-10::mtm-10, and mtm-10(ac270);Podr-1::mtm-10::SL2::GFP on UV-killed Escherichia coli OP50 (independent replicates N = 3, number of animals n = 300), control versus mtm-10(ac270), (p < 0.0001). C, quantitative real-time PCR analysis of DAF-16-dependent genes in mtm-10(ac270) animals compared with control CX5974 (parent strain) after infection with P. aeruginosa for 24 h (n = 3; three technical replicates in each case). D, representative lifespan plots of control CX5974 (parent strain) and mtm-10(ac270) animals after knockdown of daf-16 via RNAi followed by 24-h exposure to P. aeruginosa (n = 3; animals per condition = 300). mtm-10(ac270) control RNAi versus mtm-10(ac270); daf-16 RNA (p < 0.0001). RNAi, RNA interference.