Table 1.

Detail of PCR primers utilized in this study along with the corresponding annealing temperature for PCR amplification.

Genes	Reference	Primer pair with sequence (5 ${}^{\prime }$ –3 ${}^{\prime }$ ) ${}^{\mathrm{a}}$	Primer	TA ${}^{\mathrm{b}}$	Amplicon
			length (nt)	( ${}^{\circ }$ C)	size ${}^{\mathrm{c}}$ (bp)
circRNA-0100	Yin et al. (2019)	F:GCTCGGAGTTGGCATTCATC	20	57	144
		R:TCAACTAATCCGTAACAGGTCAA	20
CHD9	XM_005691972.3 in GenBank	F:ACTTGGGTGCAGAATTTGAAC	21	55	181
		R:AACTGAGAGTGTGGCGATAAC	21
Keratin 6	Yin et al. (2020)	F:CAGTCGCAGCCTCTACAACCT	21	56	159
		R:CAAATGCCACCTCCATAACCA	21
Keratin 7	Yin et al. (2020)	F:GAGTTTGTGGTGTTGAAGAA	20	56	194
		R:AAGTCCAGGGAGCGGTTGTT	20
Keratin 8	Yin et al. (2020)	F:TCCTTCAGCAGCCGCTCCTA	20	58	160
		R:CTGTAATGCCCCCCAAACCT	20
Keratin 16	Yin et al. (2020)	F:CCTTTGTGGCTAGTGGTATG	20	55	188
		R:CAGTTTCAGGGGTTGCTTAT	20
Keratin 17	Yin et al. (2020)	F:GGGGAATGGAAACAGAGGAG	20	56	112
		R:GAGGAGAGAAGCCCAAGATG	20
KLF5	KU041751 in GenBank	F:CCACCTCCATCCTATGCTGC	20	56	134
		R:TCCAAATCGGGGTTACTCCT	20
UBC	Bai et al. (2014)	F:GCATTGTTGGGTTCCTGTGT	20	52	90
		R:TTTGCATTTTGACCTGTGAG	20
YWHAZ	Bai et al. (2014)	F:TGTAGGAGCCCGTAGGTCATCT	22	56	102
		R:TTCTCTCTGTATTCTCGAGCCATCT	25
SDHA	Bai et al. (2014)	F:AGCACTGGAGGAAGCACAC	19	53	105
		R:CACAGTCGGTCTCGTTCAA	19
KLF5 ${}^{\mathrm{d}}$	The present study	F:TTAGTTTAATTTGTTAGAGAGGTTGTG	27	54	262
		R:TACCCCACRACTACTAACACTTA	23
miR-20b-5p	MIMAT0036056 in miRNAsong	F:CGCAAAGTGCTCACAGTGCAGGTAG ${}^{\mathrm{e}}$	25	63	NA
miR-29a-3p	MIMAT0036113 in miRNAsong	F:CGTAGCACCATCTGAAATCGGTT	23	61	NA
miR-153-5p	MIMAT0035983 in miRNAsong	F:CGTTGCATAGTCACAAAAGTGATC	24	60	NA
miR-24-3p	MIMAT0036091 in miRNAsong	F:CGTGGCTCAGTTCAGCAGGAAC	22	60	NA
let-7c-3p	MIMAT0035885 in miRNAsong	F:CGCTGTACAACCTTCTAGCTTTCC	24	60	NA
let-7d-5p	Bai et al. (2016)	F:CGAGAGGTAGTAGGTTGCATAGTT	24	62	NA
miR-26a-5p	Bai et al. (2016)	F:CGTTCAAGTAATCCAGGATAGGCT	24	61	NA
miR-15a-5p	Bai et al. (2016)	F:CGTAGCAGCACATAATGGTTTGTG	24	63	NA

${}^{\mathrm{a}}$  F: forward, R: reverse. ${}^{\mathrm{b}}$  TA: annealing temperature. ${}^{\mathrm{c}}$  NA: not available. ${}^{\mathrm{d}}$  KLF5 was used for the designing of BSP primers. ${}^{\mathrm{e}}$  miRNAsong: https://www2.med.muni.cz/histology/miRNAsong/index.php last access: 16 April 2021.