Table 2.

Advantages and disadvantages of biomaterials for organoid culture.

	Matrigel	Natural polymers	dECM	Synthetic polymers
Advantages	Built-in complex mixture of nutrients and proteins Readily available commercialized product Easy to handle	Inertness Low toxicity Biodegradability	Low toxicity Closely mimics native ECM High bioactivity Biodegradability	Chemically defined Xenogeneic-free Tunable mechanical and degradation properties
Disadvantages	Not clinically applicable Poorly defined Low mechanical strength Lot-to-lot variability Lacks tissue-specific ECM compositions	Low mechanical strength Lacks tissue-specific ECM compositions	Poorly defined Low mechanical strength Rapid degradation	Low bioactivity Low cell adhesion Lacks tissue-specific ECM compositions Difficult and expensive synthesis procedure