Fig. 3.

Matrigel embedding culture and morphology of early-stage brain organoids. (A) Matrigel embedding process for small or large amount of embryoid bodies (EBs) and the spin culture system. (B) The neural folding stage of hESC-derived EBs at day 6 after Matrigel embedding with single (small amount) or bulk (large amount) EBs. Scale bar=200 μm (left and right) and 500 μm (middle). (C) Brain organoids with multiple neural rosette-like structures derived from human embryonic stem cells and (day 19) and human induced pluripotent stem cells (day 20). Scale bar=500 μm.